PMN expression of intracellular cytokines after stimulation with different <i>Salmonella</i> strains. Salerno-GoncalvesRosangela KayasthaDarpan FasanoAlessio LevineMyron M. SzteinMarcelo B. 2019 <p>3-D organotypic models were exposed, or not, to either <i>Salmonella enterica</i> serovar Paratyphi A (PA), Paratyphi B (PB), or Typhi (ST) strains. After 4 hours, supernatants were collected and used to stimulate dextran-500-purified PMN. (<b>A</b>) After 4 hours of stimulation, PMN were surface and intracellular stained, and the levels of IL-6, IL-8, TNF-α and CCL3 intracellular cytokines measured by flow cytometry. PMN were gated based on their scatter characteristics and specific lineage differentiation markers: CD45+CD163-CD66c+. Numbers correspond to the % positive cells, followed by mean fluorescence intensity (MFI) in parenthesis (<i>x</i>-axis). (<b>B</b>) Bar graphs extend from the 25<sup>th</sup> to 75<sup>th</sup> percentiles; the line in the middle represents the median of the pooled data. The whiskers delineate the smallest to the largest value. The data represent 2 individual experiments with up to 3 replicates in each experiment. Horizontal lines represent significant differences (<i>P</i><0.05) between the indicated culture conditions.</p>