10.1371/journal.ppat.1003968.g003 Melvyn W. Yap Melvyn W. Yap Emily Colbeck Emily Colbeck Scott A. Ellis Scott A. Ellis Jonathan P. Stoye Jonathan P. Stoye Phylogenetic tree of <i>Fv1</i> sequences. Public Library of Science 2014 Evolutionary biology Evolutionary genetics genetics Gene function Genetic mutation microbiology Virology Viral transmission and infection Viral clearance Animal models of infection Viral replication Virulence factors and mechanisms Host-pathogen interaction 2014-03-06 04:29:56 Figure https://plos.figshare.com/articles/figure/_Phylogenetic_tree_of_Fv1_sequences_/954587 <p>The tree was generated from the open reading frames listed in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003968#ppat-1003968-t001" target="_blank">Tables 1</a> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003968#ppat-1003968-t002" target="_blank">2</a> (bases 1 to 1278 in <i>Fv1<sup>b</sup></i>) using the MegAlign programme from the DNASTAR Lasergene package. The highly divergent C-terminus was excluded from the analysis and the aspartic acid residue at position 426 (Fv1<sup>b</sup> numbering), which was the last residue conserved in all the sequences, was chosen as the cut-off point. The number of substitution events is shown at the bottom of the tree while the distances between sequence pairs is represented by the length of the branch pairs. The distance values were calculated using the Kimura distance formula that takes into account the number of non-gap mismatches and silent substitutions.</p>