Heid, Hans Rickelt, Steffen Zimbelmann, Ralf Winter, Stefanie Schumacher, Heiderose Dörflinger, Yvette Kuhn, Caecilia W. Franke, Werner Immunoelectron microscopic localization of adipophilin in briefly AIM-stimulated and OA-treated human preadipocytes. <p>(a) Cells reveal besides perilipin-positive also adipophilin-positive LDs. Localization was performed with mab AP125. A few positive, mostly smaller LDs are seen as well as plenty of non-labeled, larger LDs. Almost all LDs are closely associated and anchored with IF bundles. (b) A small, strongly immunolabeled LD is seen approaching a big, scarcely labeled LD. These LDs are obviously at the rim of combining. (c,d) Enlargements with details of filament attachment and immunolabeling sites which were highlighted by arrows (vimentin IFs) and arrowheads (adipophilin immunoreaction). Bars: a,b: 1 µm; c,d: 0.50 µm.</p> Biochemistry;lipids;Fatty acids;Lipid aggregates;Lipid metabolism;Lipid structure;metabolism;Metabolic pathways;proteins;Cytoskeletal proteins;lipoproteins;Protein interactions;Structural proteins;immunochemistry;immunology;Immunologic techniques;immunofluorescence;microscopic;localization;adipophilin;aim-stimulated;oa-treated 2014-02-28
    https://plos.figshare.com/articles/figure/_Immunoelectron_microscopic_localization_of_adipophilin_in_briefly_AIM_stimulated_and_OA_treated_human_preadipocytes_/949299
10.1371/journal.pone.0090386.g013