<i>Immunoelectron microscopic localization of perilipin in briefly AIM-stimulated human preadipocytes.</i> Hans Heid Steffen Rickelt Ralf Zimbelmann Stefanie Winter Heiderose Schumacher Yvette Dörflinger Caecilia Kuhn Werner W. Franke 10.1371/journal.pone.0090386.g012 https://plos.figshare.com/articles/figure/_Immunoelectron_microscopic_localization_of_perilipin_in_briefly_AIM_stimulated_human_preadipocytes_/949297 <p>(<b>a</b>) Shown are groups of LDs positive for perilipin by nanogold-label and silver enhancement. Localization was with mab Peri112.17. The LDs are seen closely associated and anchored with IF bundles. (<b>b</b>) Enlarged perilipin labeling with two small LDs (approximately 300–400 nm in diameter) approaching a big LD (approximately 2.0–2.5 µm in diameter) for combining and coalescence. Bundles of intermediate-sized filaments are associated to grains of the immunolabeled perilipin (arrows). Bars: 0.50 µm.</p> 2014-02-28 04:20:13 Biochemistry lipids Fatty acids Lipid aggregates Lipid metabolism Lipid structure metabolism Metabolic pathways proteins Cytoskeletal proteins lipoproteins Protein interactions Structural proteins immunochemistry immunology Immunologic techniques immunofluorescence microscopic localization perilipin aim-stimulated