%0 Figure %A Feng, Chunhong %A He, Kai %A Zhang, Chunyan %A Su, Song %A Li, Bo %A Li, Yuxiao %A Duan, Chun-Yan %A Chen, Shaokun %A Chen, Run %A Liu, Youping %A Li, Hong %A Wei, Mei %A Xia, Xianming %A Dai, Rongyang %D 2014 %T JNK/mTOR regulates GRP78 induction through ATF4 in human CCA cells. %U https://plos.figshare.com/articles/figure/_JNK_mTOR_regulates_GRP78_induction_through_ATF4_in_human_CCA_cells_/949292 %R 10.1371/journal.pone.0090388.g007 %2 https://plos.figshare.com/ndownloader/files/1404308 %K biophysics %K Protein folding %K Molecular cell biology %K Signal transduction %K Signaling cascades %K c-Jun N-terminal kinase signaling cascade %K Signaling in selected disciplines %K Oncogenic signaling %K Cellular stress responses %K Gastroenterology and hepatology %K Gastrointestinal cancers %K oncology %K Cancers and neoplasms %K Gastrointestinal tumors %K regulates %K grp78 %K induction %K atf4 %K cca %X

(A) After treated with SP600125 (SP, 20 µM) for 48 h, ATF4 and phosphorylated eIF2α in QBC939, RBE and HCCC-9810 cells were analyzed using western blot. (B) After treated with rapamycin (Rap, 20 nM) for 48 h, phosphorylated eIF2α and phosphorylated p70S6K in QBC939, RBE and HCCC-9810 cells were analyzed using western blot. (C) After treated with salubrinal (Sal, 25 µM) for 30 h with or without SP600125 (SP, 20 µM) and rapamycin (Rap, 20 nM) preincubation for 1 h, ATF4 and phosphorylated eIF2α were analyzed using western blot in HepG2 cells. (D) After treated with PF-4708671 (PF, 10 µM) and 4EGI-1 (50 µM) for 48 h, GRP78 and ATF4 in QBC939, RBE and HCCC-9810 cells were analyzed using western blot. (E) QBC939, RBE and HCCC-9810 cells were treated with rapamycin (Rap, 20 nM) for 12 h, and ATF4 and GRP78 mRNA levels were analyzed using real-time RT-PCR. Values are means±S.D. Columns, mean of three individual experiments; bars, SD. *Significantly different from control value.

%I PLOS ONE