JNK promotes the activity of mTOR in human CCA cells. Chunhong Feng Kai He Chunyan Zhang Song Su Bo Li Yuxiao Li Chun-Yan Duan Shaokun Chen Run Chen Youping Liu Hong Li Mei Wei Xianming Xia Rongyang Dai 10.1371/journal.pone.0090388.g005 https://plos.figshare.com/articles/figure/_JNK_promotes_the_activity_of_mTOR_in_human_CCA_cells_/949287 <p>(A) After treated with SP600125 (SP, 20 µM) for indicated time periods, phosphorylated p70S6K in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. Rapamycin (Rap, 20 nM)-treated cholangiocarcinoma cells were used as positive control. (B) After transfected with siJNK for 60 h, phosphorylated p70S6K in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. (C) After treated with SP600125 (SP, 20 µM) for indicated time periods, phosphorylated mTOR in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. (D) After treated with SP600125 (SP, 20 µM) for indicated time periods, phosphorylated Raptor and phosphorylated 4E-BP1 in QBC939, RBE and HCCC-9810 cells were analyzed using western blot.</p> 2014-02-28 04:18:45 biophysics Protein folding Molecular cell biology Signal transduction Signaling cascades c-Jun N-terminal kinase signaling cascade Signaling in selected disciplines Oncogenic signaling Cellular stress responses Gastroenterology and hepatology Gastrointestinal cancers oncology Cancers and neoplasms Gastrointestinal tumors mtor cca