%0 Figure %A Heid, Hans %A Rickelt, Steffen %A Zimbelmann, Ralf %A Winter, Stefanie %A Schumacher, Heiderose %A Dörflinger, Yvette %A Kuhn, Caecilia %A W. Franke, Werner %D 2014 %T Summary scheme and brief description of stimulation methods used for adipose conversion and for the generation of different, distinct types of lipid droplets. %U https://plos.figshare.com/articles/figure/_Summary_scheme_and_brief_description_of_stimulation_methods_used_for_adipose_conversion_and_for_the_generation_of_different_distinct_types_of_lipid_droplets_/949281 %R 10.1371/journal.pone.0090386.g003 %2 https://plos.figshare.com/ndownloader/files/1404297 %K Biochemistry %K lipids %K Fatty acids %K Lipid aggregates %K Lipid metabolism %K Lipid structure %K metabolism %K Metabolic pathways %K proteins %K Cytoskeletal proteins %K lipoproteins %K Protein interactions %K Structural proteins %K immunochemistry %K immunology %K Immunologic techniques %K immunofluorescence %K stimulation %K methods %K adipose %K types %K lipid %X

Major treatments and involved PLIN proteins are shown. Preadipocytes containing many small LDs are not differentiated for several weeks with AIM containing media as conventionally described (top row; cp. Fig. S1), but only very briefly (1–3 days), giving rise to “Adipocytes” (boxed area). Additional, short OA-treatment leads to “OA-Adipocytes” (boxed area, right side). Treatment with OA only - without AIM stimulation - leads to “OA-Preadipocytes” (bottom). Note the huge heterogeneity of sizes and colors of LDs seen within “OA-Adipocytes”. LDs are endogenously generated at the endoplasmic reticulum and stained positively for perilipin (“Endogenous-LDs”, red). Other LDs are obtained from the exogenous uptake of OA and stained positively for adipophilin, TIP47 and S3-12 (“Exogenous-LDs”, green). Merged LDs by fusion and mixed-type expression are seen by yellow color. The backway arrows indicate possible routes of LDs during lipolysis.

%I PLOS ONE