Effect of Sirtinol, a specific SIRT-1 inhibitor, on H<sub>2</sub>S-exerted protection against ER stress induced by formaldehyde. Xiang Li Kai-Yan Zhang Ping Zhang Li-Xun Chen Li Wang Ming Xie Chun-Yan Wang Xiao-Qing Tang 10.1371/journal.pone.0089856.g004 https://plos.figshare.com/articles/figure/_Effect_of_Sirtinol_a_specific_SIRT_1_inhibitor_on_H_2_S_exerted_protection_against_ER_stress_induced_by_formaldehyde_/948942 <p>PC12 cells preincubated with Sirtinol (15 µM) 30 min before pretreatment with NaHS (200 µM) for 30 min prior to 24-h exposure of formaldehyde (FA, 120 µM). The levels of GRP78 (A), CHOP (B), and cleaved caspase-12 (C) expression in PC12 cells were detected by Western blot using anti-GRP78, -CHOP, and -cleaved caspase-12 antibody, respectively. In all blots, staining for β-actin was used as a loading control. Values are the mean ± S.E.M. of three independent experiments. ***<i>P</i><0.001, <i>vs</i> control group; <sup>###</sup><i>P</i><0.001, <i>vs</i> FA-treated alone group; <sup>$$</sup><i>P</i><0.01, <i>vs</i> cotreated with NaHS and FA group.</p> 2014-02-28 03:58:55 Biochemistry proteins Molecular cell biology Cellular structures Subcellular organelles cytometry flow cytometry neuroscience Neurochemistry neuromodulation toxicology Toxic agents Environmental chemistry pollutants neurology Neuropharmacology sirt-1 er induced