10.1371/journal.pone.0089856.g003 Xiang Li Xiang Li Kai-Yan Zhang Kai-Yan Zhang Ping Zhang Ping Zhang Li-Xun Chen Li-Xun Chen Li Wang Li Wang Ming Xie Ming Xie Chun-Yan Wang Chun-Yan Wang Xiao-Qing Tang Xiao-Qing Tang Effect of H<sub>2</sub>S on the expression of SIRT-1 in PC12 cells. Public Library of Science 2014 Biochemistry proteins Molecular cell biology Cellular structures Subcellular organelles cytometry flow cytometry neuroscience Neurochemistry neuromodulation toxicology Toxic agents Environmental chemistry pollutants neurology Neuropharmacology sirt-1 pc12 2014-02-28 03:58:55 Figure https://plos.figshare.com/articles/figure/_Effect_of_H_2_S_on_the_expression_of_SIRT_1_in_PC12_cells_/948940 <p>A, PC12 cells were treated with different concentrations of NaHS (100, 200 and 400 µmol/L) for 24 h. B, PC12 cells pretreated with NaHS at a concentration of 200 µM for 30 min prior to 24-h exposure of formaldehyde (FA, 120 µM). The levels of SIRT-1 expression in PC12 cells were detected by Western blot using anti-SIRT-1 antibody. In all blots, staining for β-actin was used as a loading control. Values are the mean ± S.E.M. of three independent experiments. *<i>P</i><0.05; **<i>P</i><0.01; ***<i>P</i><0.001, <i>vs</i> control group; <sup>#</sup><i>P</i><0.05, <i>vs</i> FA-treated alone group.</p>