Subcellular localization of mPB and NP-mPB.
Jin-Bon Hong
Fu-Ju Chou
Amy T. Ku
Hsiang-Hsuan Fan
Tung-Lung Lee
Yung-Hsin Huang
Tsung-Lin Yang
I-Chang Su
I-Shing Yu
Shu-Wha Lin
Chung-Liang Chien
Hong-Nerng Ho
You-Tzung Chen
10.1371/journal.pone.0089396.g007
https://plos.figshare.com/articles/figure/_Subcellular_localization_of_mPB_and_NP_mPB_/943408
<p>Hela cells were transfected with mPB-tGFP or NP-mPB-tGFP expression vectors and immunostained with an anti-cyclin T1 antibody (red) and an anti-fibrillarin antibody (blue). (A) Fluorescence microscopy revealed that mPB was localized in the nucleus, but largely outside the nucleoli, as indicated by the fibrillarin. The cyclin T1 signal was dotted and spared the nucleus. (B) In contrast, NP-mPB was localized in the nucleus, with a strong staining in the nucleoli. There was a dotted pattern of NP-mPB in the nucleus beside the nucleoli. Some of the dots were overlapped with the cyclin T1 signal.</p>
2014-02-24 03:36:18
biotechnology
Genetic engineering
genetics
Human genetics
Gene therapy
genomics
Functional genomics
Molecular cell biology
Cellular structures
nucleolus
Cellular types
stem cells
Embryonic stem cells
transposons
DNA transposons
localization
mpb