10.1371/journal.pone.0089396.g003 Jin-Bon Hong Jin-Bon Hong Fu-Ju Chou Fu-Ju Chou Amy T. Ku Amy T. Ku Hsiang-Hsuan Fan Hsiang-Hsuan Fan Tung-Lung Lee Tung-Lung Lee Yung-Hsin Huang Yung-Hsin Huang Tsung-Lin Yang Tsung-Lin Yang I-Chang Su I-Chang Su I-Shing Yu I-Shing Yu Shu-Wha Lin Shu-Wha Lin Chung-Liang Chien Chung-Liang Chien Hong-Nerng Ho Hong-Nerng Ho You-Tzung Chen You-Tzung Chen Evaluation of the transposition efficiency mediated by the gradient concentrations of PBase. Public Library of Science 2014 biotechnology Genetic engineering genetics Human genetics Gene therapy genomics Functional genomics Molecular cell biology Cellular structures nucleolus Cellular types stem cells Embryonic stem cells transposons DNA transposons transposition mediated gradient concentrations 2014-02-24 03:36:18 Figure https://plos.figshare.com/articles/figure/_Evaluation_of_the_transposition_efficiency_mediated_by_the_gradient_concentrations_of_PBase_/943403 <p>(A) The NP-mPB group demonstrated more colonies compared to the mPB group in HEK293T cells transfected with varying amounts of plasmid DNA. (B) Colony numbers were quantified, revealing a 2.5- to 6-fold increase in transposon integration efficiency for NP-mPB. The transposition efficiency was higher when more plasmid was transfected for both groups. No overproduction inhibition was noted. n  = 3 for each condition; bars indicate mean ± standard error; ** <i>P</i><0.01, *** <i>P</i><0.001.</p>