10.1371/journal.pone.0089810.g007 Daniela Farkas Daniela Farkas Donatas Kraskauskas Donatas Kraskauskas Jennifer I. Drake Jennifer I. Drake Aysar A. Alhussaini Aysar A. Alhussaini Vita Kraskauskiene Vita Kraskauskiene Harm J. Bogaard Harm J. Bogaard Carlyne D. Cool Carlyne D. Cool Norbert F. Voelkel Norbert F. Voelkel Laszlo Farkas Laszlo Farkas Proliferation and apoptosis in the lungs of AMD3100-treated SU5416/chronic hypoxia (SuHx) animals. Public Library of Science 2014 Anatomy and physiology cardiovascular system Circulatory physiology Model organisms Animal models rat Molecular cell biology Cellular types Endothelial cells cardiovascular Pulmonary vascular diseases Vascular biology apoptosis lungs amd3100-treated hypoxia 2014-02-24 03:32:53 Figure https://plos.figshare.com/articles/figure/_Proliferation_and_apoptosis_in_the_lungs_of_AMD3100_treated_SU5416_chronic_hypoxia_SuHx_animals_/943324 <p>(<b>A</b>) Representative Western blot for proliferating cell nuclear antigen (PCNA) and cleaved caspase-3 in the lung tissue protein lysate of naïve control animals (n = 3), SuHx + vehicle (n = 6) and SuHx + AMD3100 treated rats (n = 6). β-actin was used as loading control. (<b>B-C</b>) Densitometric analysis indicates increased PCNA (B) and cleaved caspase-3 (C) protein levels in the lungs of SuHx + vehicle animals vs. controls, and that AMD3100 treatment significantly reduced PCNA and cleaved caspase-3 protein levels in the lungs of SuHx animals. Densitometric values were normalized vs. β-actin and expressed as n-fold of naïve controls. n = 3 animals per group for controls and n = 6 animals per group for SuHx + vehicle and SuHx + AMD3100 groups. * <i>P</i><0.05 and ** <i>P</i><0.01.</p>