K118I/K120P CD28 binds CD80 with high avidity in the absence of TCR signaling. Mariano Sanchez-Lockhart Ana V. Rojas Margaret M. Fettis Richard Bauserman Trissha R. Higa Hongyu Miao Richard E. Waugh Jim Miller 10.1371/journal.pone.0089263.g004 https://plos.figshare.com/articles/figure/_K118I_K120P_CD28_binds_CD80_with_high_avidity_in_the_absence_of_TCR_signaling_/943318 <p>Cell frequency adhesion of CD28-deficient, DO11.10 T cells retrovirally transduced with WT CD28-YFP or K118I/K120P CD28-YFP to control beads (None) or beads coated with CD80 in the absence (Unstim) and after (Anti-CD3) stimulation. Cell adhesion data are presented as mean ± SD of the adhesion frequency for individual cells (25 impingements each; n = 6–7 for control beads and 12–14 for CD80-Fc beads; p values for t tests between samples are shown).</p> 2014-02-24 03:32:16 Biochemistry proteins Immune system proteins Protein interactions protein structure T-cell receptors Transmembrane proteins Biomacromolecule-ligand interactions Macromolecular assemblies biophysics immunology Immune cells t cells Immune response immunomodulation Molecular cell biology Signal transduction Mechanisms of signal transduction crosstalk Membrane receptor signaling Immunologic receptor signaling Signaling in cellular processes Transmembrane signaling cell adhesion Computer modeling Computerized simulations cd28 binds cd80 avidity tcr