10.1371/journal.pone.0089810.g001
Daniela Farkas
Daniela
Farkas
Donatas Kraskauskas
Donatas
Kraskauskas
Jennifer I. Drake
Jennifer
I. Drake
Aysar A. Alhussaini
Aysar
A. Alhussaini
Vita Kraskauskiene
Vita
Kraskauskiene
Harm J. Bogaard
Harm
J. Bogaard
Carlyne D. Cool
Carlyne
D. Cool
Norbert F. Voelkel
Norbert
F. Voelkel
Laszlo Farkas
Laszlo
Farkas
Accumulation of c-kit<sup>+</sup> cells in the SU5416/chronic hypoxia (SuHx) model.
Public Library of Science
2014
Anatomy and physiology
cardiovascular system
Circulatory physiology
Model organisms
Animal models
rat
Molecular cell biology
Cellular types
Endothelial cells
cardiovascular
Pulmonary vascular diseases
Vascular biology
cells
hypoxia
2014-02-24 03:32:53
Figure
https://plos.figshare.com/articles/figure/_Accumulation_of_c_kit_cells_in_the_SU5416_chronic_hypoxia_SuHx_model_/943311
<p>(<b>A</b>) Representative images of <i>in situ</i> hybridization images demonstrating <i>Kit</i> mRNA expression. In naïve control animals, occasional low <i>Kit</i> expression was found in cells of vessels and alveolar walls<b>,</b> whereas multiple cells in lumen-obliterating regions and vessel wall/perivascular region expressed <i>Kit</i> (granular staining pattern) in the lung of a SuHx animal. The open arrows indicate cells with strong <i>Kit</i> expression. Counterstaining with Gill’s Hematoxylin. Magnification: 400×. Scale bar: 20 µm. (<b>B</b>) Quantification of the number of c-kit<sup>+</sup> cells/vessel over time. n = 3 animals per group. * <i>P</i><0.05, ** <i>P</i><0.01. (<b>C</b>) Images demonstrate representative optical sections (confocal microscopy). c-kit<sup>+</sup>, c-kit<sup>+</sup> von Willebrand Factor<sup>+</sup> (vWF<sup>+</sup>) and c-kit<sup>+</sup> α-smooth muscle actin<sup>+</sup> (α-SMA<sup>+</sup>) cells were occasionally found in alveolar walls and vessel walls of naïve control animals. c-kit<sup>+</sup> and c-kit<sup>+</sup> α-SMA<sup>+</sup> cells accumulated in and around the pulmonary arteries of SuHx animals over time. The number of c-kit<sup>+</sup> vWF<sup>+</sup> cells also increased until day 21 in and around the pulmonary arteries of SuHx animals and started to decline thereafter. Arrows indicate c-kit<sup>+</sup> vWF<sup>+</sup> or c-kit<sup>+</sup> α-SMA<sup>+</sup> cells. Nuclear counterstaining with 4',6-diamidino-2-phenylindole (DAPI). Magnification: 630×. Scale bar: 20 µm. (<b>D-E</b>) Quantification of the number of c-kit<sup>+</sup> vWF<sup>+</sup> (D) and c-kit<sup>+</sup> α-SMA<sup>+</sup> (E) cells per vessel. n = 3 animals per group. * <i>P</i><0.05, ** <i>P</i><0.01 and *** <i>P</i><0.0001.</p>