%0 Figure %A L. Van Sinderen, Michelle %A Steinberg, Gregory R. %A B. Jørgensen, Sebastian %A Q. To, Sarah %A C. Knower, Kevin %A D. Clyne, Colin %A Honeyman, Jane %A D. Chow, Jenny %A A. Herridge, Kerrie %A E. E. Jones, Margaret %A R. Simpson, Evan %A Chin Boon, Wah %D 2014 %T Real-time PCR analysis of mRNA lipid profile in the liver. %U https://plos.figshare.com/articles/figure/Real_time_PCR_analysis_of_mRNA_lipid_profile_in_the_liver_/929340 %R 10.1371/journal.pone.0087230.g007 %2 https://plos.figshare.com/ndownloader/files/1380094 %K Anatomy and physiology %K Endocrine system %K Endocrine physiology %K insulin %K Reproductive endocrinology %K Diabetic endocrinology %K Biochemistry %K hormones %K Model organisms %K Animal models %K mouse %K Endocrinology %K pcr %K mrna %K lipid %X

Real-time-PCR analyses of Fasn, ACCα and Scd-1 mRNA expression were performed on cDNA derived from total RNA prepared from fasted liver tissue of (a) 3 and (b) 6 month-old male wildtype (WT, n = 8), aromatase knockout (KO, n = 8) and 2.5 µg/day 17β-estradiol-treated KO (KOE, n = 7) mice. Expression data from 7–8 samples per genotype are shown following normalization for cyclophilin mRNA expression, and presented from replicate analysis as the mean ± SD. *p<0.05, **p<0.01 and **p<0.001, versus expression in age-matched WT samples and ##p<0.01, ###p<0.001 versus KO samples.

%I PLOS ONE