10.1371/journal.pone.0087230.g007 Michelle L. Van Sinderen Michelle L. Van Sinderen Gregory R. Steinberg Gregory R. Steinberg Sebastian B. Jørgensen Sebastian B. Jørgensen Sarah Q. To Sarah Q. To Kevin C. Knower Kevin C. Knower Colin D. Clyne Colin D. Clyne Jane Honeyman Jane Honeyman Jenny D. Chow Jenny D. Chow Kerrie A. Herridge Kerrie A. Herridge Margaret E. E. Jones Margaret E. E. Jones Evan R. Simpson Evan R. Simpson Wah Chin Boon Wah Chin Boon Real-time PCR analysis of mRNA lipid profile in the liver. Public Library of Science 2014 Anatomy and physiology Endocrine system Endocrine physiology insulin Reproductive endocrinology Diabetic endocrinology Biochemistry hormones Model organisms Animal models mouse Endocrinology pcr mrna lipid 2014-02-10 03:23:23 Figure https://plos.figshare.com/articles/figure/Real_time_PCR_analysis_of_mRNA_lipid_profile_in_the_liver_/929340 <p>Real-time-PCR analyses of Fasn, ACCα and Scd-1 mRNA expression were performed on cDNA derived from total RNA prepared from fasted liver tissue of (a) 3 and (b) 6 month-old male wildtype (WT, n = 8), aromatase knockout (KO, n = 8) and 2.5 µg/day 17β-estradiol-treated KO (KOE, n = 7) mice. Expression data from 7–8 samples per genotype are shown following normalization for cyclophilin mRNA expression, and presented from replicate analysis as the mean ± SD. <i>*p&lt;0.05, **p&lt;0.01 and **p&lt;0.001</i>, versus expression in age-matched WT samples and <i><sup>##</sup>p&lt;0.01, <sup>###</sup>p&lt;0.001</i> versus KO samples.</p>