10.1371/journal.pone.0087230.g007
Michelle L. Van Sinderen
Michelle
L. Van Sinderen
Gregory R. Steinberg
Gregory R.
Steinberg
Sebastian B. Jørgensen
Sebastian
B. Jørgensen
Sarah Q. To
Sarah
Q. To
Kevin C. Knower
Kevin
C. Knower
Colin D. Clyne
Colin
D. Clyne
Jane Honeyman
Jane
Honeyman
Jenny D. Chow
Jenny
D. Chow
Kerrie A. Herridge
Kerrie
A. Herridge
Margaret E. E. Jones
Margaret
E. E. Jones
Evan R. Simpson
Evan
R. Simpson
Wah Chin Boon
Wah
Chin Boon
Real-time PCR analysis of mRNA lipid profile in the liver.
Public Library of Science
2014
Anatomy and physiology
Endocrine system
Endocrine physiology
insulin
Reproductive endocrinology
Diabetic endocrinology
Biochemistry
hormones
Model organisms
Animal models
mouse
Endocrinology
pcr
mrna
lipid
2014-02-10 03:23:23
Figure
https://plos.figshare.com/articles/figure/Real_time_PCR_analysis_of_mRNA_lipid_profile_in_the_liver_/929340
<p>Real-time-PCR analyses of Fasn, ACCα and Scd-1 mRNA expression were performed on cDNA derived from total RNA prepared from fasted liver tissue of (a) 3 and (b) 6 month-old male wildtype (WT, n = 8), aromatase knockout (KO, n = 8) and 2.5 µg/day 17β-estradiol-treated KO (KOE, n = 7) mice. Expression data from 7–8 samples per genotype are shown following normalization for cyclophilin mRNA expression, and presented from replicate analysis as the mean ± SD. <i>*p<0.05, **p<0.01 and **p<0.001</i>, versus expression in age-matched WT samples and <i><sup>##</sup>p<0.01, <sup>###</sup>p<0.001</i> versus KO samples.</p>