10.1371/journal.pone.0088365.g005
Slobodan Culina
Slobodan
Culina
François-Xavier Mauvais
François-Xavier
Mauvais
Hsiang-Ting Hsu
Hsiang-Ting
Hsu
Anne Burgevin
Anne
Burgevin
Suzanne Guénette
Suzanne
Guénette
Anna Moser
Anna
Moser
Peter van Endert
Peter van
Endert
HeLa Tet-on cells expressing H-2K<sup>b</sup> were nucleofected with 4×100 nM siRNA.
Public Library of Science
2014
Anatomy and physiology
Immune physiology
Immune cells
Biochemistry
enzymes
Enzyme classes
hydrolases
metabolism
Protein metabolism
immunology
Antigen-presenting cells
Antigen processing and recognition
Immune response
Immune system
immunity
Major histocompatibility complex
Model organisms
Animal models
Animal types
Laboratory animals
tet-on
cells
expressing
nucleofected
nm
2014-02-07 14:45:56
Figure
https://plos.figshare.com/articles/figure/_HeLa_Tet_on_cells_expressing_H_2K_b_were_nucleofected_with_4_215_100_nM_siRNA_/928416
<p>Twenty-four hours later, the cells were electroporated with inducible plasmids encoding amyloid beta preceded by a signal peptide (+SP) or not (−SP), or encoding proinsulin, all tagged with the S8L peptide at the C-terminus. Protein expression was induced immediately by addition of 1 µg/ml doxycylin. Forty-eight hours later, K<sup>b</sup>/S8L complexes on the cell surface were detected using mAb 25D1.16 (Ab1) followed by FITC-labeled goat anti-mouse Abs (Ab2) and Alexa488-labeled goat anti-FITC Ab (Ab3). Control samples were HeLa-K<sup>b</sup> cells pulsed for 2 h with 10<sup>−8 </sup>M S8L and stained with Abs 1, 2 and 3 or with Abs 2 and 3 only, as well as peptide-pulsed HeLa cells expressing H-2K<sup>d</sup> stained with Abs 1, 2 and 3. One of two experiments is shown.</p>