10.1371/journal.pone.0088365.g004 Slobodan Culina Slobodan Culina François-Xavier Mauvais François-Xavier Mauvais Hsiang-Ting Hsu Hsiang-Ting Hsu Anne Burgevin Anne Burgevin Suzanne Guénette Suzanne Guénette Anna Moser Anna Moser Peter van Endert Peter van Endert Role of IDE in expression of “proteasome-independent” HLA class I alleles. Public Library of Science 2014 Anatomy and physiology Immune physiology Immune cells Biochemistry enzymes Enzyme classes hydrolases metabolism Protein metabolism immunology Antigen-presenting cells Antigen processing and recognition Immune response Immune system immunity Major histocompatibility complex Model organisms Animal models Animal types Laboratory animals ide hla 2014-02-07 14:45:56 Figure https://plos.figshare.com/articles/figure/_Role_of_IDE_in_expression_of_8220_proteasome_independent_8221_HLA_class_I_alleles_/928415 <p>HLA-A3-expressing HEK293 cells and HLA-B27-transfected HeLa cells were transfected with 4×100 nM nM siRNA. Sixty-five hours later, 1 µM epoxomicin or 10 µg/ml brefeldin A were added to the cultures for 2 h or 30 min, respectively, before removal of most cell surface class I molecules by acid stripping and another 6 h incubation with the same drugs. HLA class I expression was detected using mAb W6/32 immediately after acid stripping (<b>A</b>, <b>C</b> left panel) and after the 6 h incubation (<b>A</b>, <b>C</b> center panel). The panels on the right show HLA class I re-expression relative to untreated cells as histograms. The panels in <b>B</b> and <b>D</b> show an evaluation of HEK293 cells using mAb GAP.A3 with specificity for HLA-A3 (<b>B</b>) and of HeLa-B27 cells using mAb ME-1 recognizing HLA-B27 (<b>D</b>). AS, acid stripping; BFA, brefeldin A; EPO, epoxomicin; filled histogram, secondary Ab only. One out of 3 experiments is shown.</p>