Bhaskar, Ashima Chawla, Manbeena Mehta, Mansi Parikh, Pankti Chandra, Pallavi Bhave, Devayani Kumar, Dhiraj Carroll, Kate S. Singh, Amit Redox heterogeneity in drug-resistant patient isolates during infection. <p>Infection of THP-1 macrophages, confocal microscopy, and <i>E<sub>MSH</sub></i> measurements were performed as described for <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003902#ppat-1003902-g006" target="_blank">Figure 6</a>. False color ratio confocal image of Jal 2287 (A) and MYC 431 (E) inside THP-1 at 24 h p.i. Small dashed line boxes indicate bacilli with varying <i>E<sub>MSH</sub></i>. Co-localization of <i>Mtb</i> in (B and F) endosomes, (C and G) lysosomes, and (D and H) autophagosomes. False color ratio images were generated as described in SI <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003902#s4" target="_blank"><i>Materials and Methods</i></a>. Small dashed line boxes indicate co-localized bacilli and large solid line boxes represent the enlarged view of one of the co-localized bacilli. Relative distribution of <i>Mtb</i> subpopulations with varying <i>E<sub>MSH</sub></i> is depicted as scattered plots and stacked bar graphs. Each point on the plot represents a bacterium. Approximately, ≥80 individual bacilli within macrophages and ≥50 individual bacilli within each compartment were imaged to calculate <i>E<sub>MSH</sub></i>. Color bar corresponds to the 405/488 nm ratios ranging from 0 to 1. Data shown is representative of at least three independent experiments.</p> Biochemistry;biotechnology;microbiology;Infectious diseases;heterogeneity;drug-resistant;isolates 2014-01-30
    https://plos.figshare.com/articles/figure/_Redox_heterogeneity_in_drug_resistant_patient_isolates_during_infection_/918779
10.1371/journal.ppat.1003902.g007