Schmidt, Frank Kakar, Niamatullah Meyer, Tanja C. Depke, Maren Masouris, Ilias Burchhardt, Gerhard Gómez-Mejia, Alejandro Dhople, Vishnu Håvarstein, Leiv S. Sun, Zhi Moritz, Robert L. Völker, Uwe Koedel, Uwe Hammerschmidt, Sven Identification of ComDE and AliB in <i>S</i>. <i>pneumoniae</i> D39 from <i>in vitro</i> culture or <i>in vivo</i> infection samples. <p><b>(A)</b> Voronoi Treemaps of predicted and detected pneumococcal proteins. <i>S</i>. <i>pneumoniae</i> D39 was cultured under various <i>in vitro</i> conditions, and a map of <i>in vitro</i> identified proteins was generated (left panel). Pneumococcal proteins identified in control reactions with a trypsin-digestion of pneumococci on a filter (middle panel). These pneumococci represent bacteria from a culture that was also used to infect mice. Proteins identified in pneumococci recovered from the CSF of mice (n = 5 samples each of 4 mice), enriched by sequential centrifugation on a filter, and digested by trypsin (right panel). Gray spheroids represent annotated protein entries from the SEED with light gray not identified in sample and dark gray never identified. Orange spheroids represent identified proteins by MS. <b>(B)</b> Identified proteins are depicted in enlarged regions of Voronoi treemaps. ComD, ComE (upper left), and AliB (lower left) were not identified in <i>S</i>. <i>pneumoniae</i> D39 from <i>in vitro</i> culture samples, while all three proteins were identified in samples from <i>in vivo</i> infection (upper and lower right).</p> competence regulon;system ComDE;meningitis mice;AliB oligopeptide transporter;pneumococcal meningitis Streptococcus pneumoniae;cisterna magna;virulence factors;pneumococcal adaptation;mass spectrometry;spectra library;meningeal pathogens;CSF;proteome analysis;pneumococcal meningitis;niche-specific analysis;cerebrospinal fluid;MS;protein antigens;eukaryotic proteins;685 pneumococci proteins;vivo proteome;attenuated meningeal inflammation;oligopeptide transporter system;vivo proteomics;mouse meningitis model;disease severity;host compartment;wild-type pneumococci;filter controls;proteome-based approach;substrate-binding protein AliB 2019-07-29
    https://plos.figshare.com/articles/figure/Identification_of_ComDE_and_AliB_in_i_S_i_i_pneumoniae_i_D39_from_i_in_vitro_i_culture_or_i_in_vivo_i_infection_samples_/9161699
10.1371/journal.ppat.1007987.g002