Phenotype of <i>OsGA2ox5</i>-overexpressing plants.
Chi Shan
Zhiling Mei
Jianli Duan
Haiying Chen
Huafeng Feng
Weiming Cai
10.1371/journal.pone.0087110.g003
https://plos.figshare.com/articles/figure/_Phenotype_of_OsGA2ox5_overexpressing_plants_/914523
<p>(A) Phenotype of WT (left) and dwarf <i>OsGA2ox5-ox</i> plants (right). 2-week-old water cultured seedlings were used for photograph; (B) Arrows indicate the boundary between the second leaf sheath and the blade of 5-day-old water cultured seedlings. Bar = 1 cm; (C) Longitudinal sections of the elongated regions of the second leaf sheath of WT (left) and <i>OsGA2ox5-ox</i> plants (right). Bar = 25 µm; (D) Quantitative measurement of the cell length of second leaf sheath in WT and OX (n = 20). Error bars show standard errors (SE). Asterisks indicated significant differences at P <0.01 compared with the wild type by Student’s t test; (E) Ectopic expression of <i>OsGA2ox5</i> in <i>Arabidopsis.</i> Left is <i>OsGA2ox5</i> transgenic plants and wide type <i>Arabidopsis</i> (Col) is on the right. Plants photographed are 4-weeks-old. Bar = 2.5 cm; (F) Expression level of <i>OsOsGA2ox5</i> in transgenic rice; WT was used as a control; (G) Southern blotting analysis of transgenic plants. Restriction endonuclease <i>Hind III</i> was used to digest the genomic DNA from the leaf tissue. M, molecular marker; WT, wild type; L7, L12, L13, three transgenic lines.</p>
2014-01-27 03:50:35
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