10.1371/journal.pone.0219313.g002
Anja Sophie Schmid
Anja Sophie
Schmid
Dario Neri
Dario
Neri
SEC complexation experimental scheme and positive control.
Public Library of Science
2019
SPR
size exclusion chromatography
surface plasmon resonance
IL 15-based biopharmaceuticals
IL 15R subunits
bind IL 15.
mouse IL 15R
mouse IL 15
F 8IL
IL 15
EDA
cancer therapy applications
SEC
IL 15 complexation
cell-based activity assay none
IL 15R fusions
novel interleukin -15 receptor
interleukin -15 complexation Interleukin -15
F 8 antibody
fusion protein F 8IL
IL 15R fusion proteins
fusion protein
2019-07-26 17:25:06
Figure
https://plos.figshare.com/articles/figure/SEC_complexation_experimental_scheme_and_positive_control_/9117386
<p>(A+B) Scheme of the SEC complexation experiments visualizing (A) IL15Rα binding to IL15 by showing (B) the merging of the separate protein peaks into one single complex peak. (C) Positive control experimental scheme of F8IL15Rα binding to its cognate antigen EDA and (D) experimental data showing peak merging at equimolar amounts of individual complex components. EDA = extra-domain A of fibronectin, F8Ab = F8 antibody.</p>