10.1371/journal.pone.0219313.g002 Anja Sophie Schmid Anja Sophie Schmid Dario Neri Dario Neri SEC complexation experimental scheme and positive control. Public Library of Science 2019 SPR size exclusion chromatography surface plasmon resonance IL 15-based biopharmaceuticals IL 15R subunits bind IL 15. mouse IL 15R mouse IL 15 F 8IL IL 15 EDA cancer therapy applications SEC IL 15 complexation cell-based activity assay none IL 15R fusions novel interleukin -15 receptor interleukin -15 complexation Interleukin -15 F 8 antibody fusion protein F 8IL IL 15R fusion proteins fusion protein 2019-07-26 17:25:06 Figure https://plos.figshare.com/articles/figure/SEC_complexation_experimental_scheme_and_positive_control_/9117386 <p>(A+B) Scheme of the SEC complexation experiments visualizing (A) IL15Rα binding to IL15 by showing (B) the merging of the separate protein peaks into one single complex peak. (C) Positive control experimental scheme of F8IL15Rα binding to its cognate antigen EDA and (D) experimental data showing peak merging at equimolar amounts of individual complex components. EDA = extra-domain A of fibronectin, F8Ab = F8 antibody.</p>