Localization of αB-crystallin in rat heart tissue before and after heat stress <i>in vivo</i>. Shu Tang Yingjun Lv Hongbo Chen Abdelnasir Adam Yanfen Cheng Jörg Hartung Endong Bao 10.1371/journal.pone.0086937.g003 https://plos.figshare.com/articles/figure/_Localization_of_B_crystallin_in_rat_heart_tissue_before_and_after_heat_stress_in_vivo_/908673 <p>Immunohistochemical staining, scale bar = 10 µm, αB-crystallin (brown color, DAB). (a) Positive αB-crystallin signals were localized in the cytoplasm of non-stressed rat heart cells. In heat-stressed rat hearts, the following were observed at the indicated time points: (b) 20 min, αB-crystallin signals were positive mainly in the cytoplasm but weaker compared to the control group; (c) 40 min, αB-crystallin signals were still detected in the cytoplasm but stronger than at 20 min of heat stress; (d) 60 min, αB-crystallin was still localized in the cytoplasm; (e) 80 min, weaker positive αB-crystallin signals were still mainly expressed in the cytoplasm; (f) 100 min, αB-crystallin was expressed weakly in the cytoplasm.</p> 2014-01-22 03:50:37 Computational biology Molecular genetics gene expression genetics Model organisms Animal models rat Molecular cell biology Cellular stress responses proteomics Diagnostic medicine pathology General pathology biomarkers Animal management Veterinary pathology