Production of chemokines by APC infected with recombinant LASV. Delphine Pannetier Stéphanie Reynard Marion Russier Xavier Carnec Sylvain Baize 10.1371/journal.pntd.0002637.g005 https://plos.figshare.com/articles/figure/_Production_of_chemokines_by_APC_infected_with_recombinant_LASV_/898687 <p>The production of CC and CXC chemokines by DC (A, B) and MP (C, D) was assessed after mock infection (white bars), or infection with recombinant wild-type LASV (gray bars) or rLASV NP-D389A/G392A (rNP LASV) (black bars). (A, C) The synthesis of mRNAs was analyzed by RT-qPCR 24 h after infection. (B, D) The protein levels released in the supernatants were quantified by ELISA 24 h (only for CXCL9, 10 and 11) and 48 h after infection. <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002637#s3" target="_blank">Results</a> are expressed as the mean ± SE of 4 (A, C) and 3–7 (B, D) independent experiments. Significant differences are indicated as follows: * (<i>p</i><0.05), ** (<i>p</i><0.01) and *** (p<0.001).</p> 2014-01-09 03:38:02 immunology Immune cells Antigen-presenting cells immunity Immunity to infections Innate immunity Immune response microbiology Virology Animal models of infection Emerging viral diseases Emerging infectious diseases Host-pathogen interaction chemokines apc infected recombinant