Rahman, Hossinur M. King, Rebecca K. Shewell, Lucy A. Semchenko, Evgeny E. Hartley-Tassell, Lauren Wilson, Jennifer C. J. Day, Christopher Korolik, Victoria Analysis of protein interactions with Tlp<sup>sig</sup><sub>2.3.4</sub> using the yeast two-hybrid system. <p>Testing for interactions of Tlp<sup>sig</sup><sub>2.3.4</sub> fused to the GAL-4 activation domain (AD- Tlp<sup>sig</sup><sub>2.3.4</sub>) and GAL-4 binding domain (BD- Tlp<sup>sig</sup><sub>2.3.4</sub>) with all chemotaxis proteins and individual domains fused to the GAL-4 DNA binding domain and GAL-4 activation domain respectively. Co-transformation of AD-CheW and BD-CheA was used as a positive control when testing for interactions, with a result of +++.</p><p>+++: cream, dense growth of >75% of co-transformants observed on high stringency media and intermediate stringency media.</p><p>++: cream − pink moderate growth for ∼75% or more of co-transformants observed on high stringency media and intermediate stringency media.</p><p>+: cream − pink light growth for ∼50% or more of co-transformants observed on high stringency media and/or intermediate stringency media.</p><p>0: no growth of co-transformants observed.</p> interactions;yeast;two-hybrid 2014-01-02
    https://plos.figshare.com/articles/dataset/_Analysis_of_protein_interactions_with_Tlp_sig_2_3_4_using_the_yeast_two_hybrid_system_/892431
10.1371/journal.ppat.1003822.t002