10.1371/journal.pone.0084060.g001 Kristen J. Holmes Kristen J. Holmes Daniel M. Klass Daniel M. Klass Evan L. Guiney Evan L. Guiney Martha S. Cyert Martha S. Cyert Whi3 localizes to stress granules during glucose deprivation. Public Library of Science 2013 Biochemistry Nucleic acids rna Macromolecular assemblies genetics Gene function Molecular genetics Model organisms Yeast and fungal models Saccharomyces cerevisiae Molecular cell biology Cellular stress responses Cellular structures gene expression localizes granules glucose 2013-12-27 02:42:57 Figure https://plos.figshare.com/articles/figure/_Whi3_localizes_to_stress_granules_during_glucose_deprivation_/887418 <p>(A) Cells coexpressing Whi3-GFP and stress granule marker Pub1-mCherry were deprived of glucose for 10 minutes. Glucose deprivation caused Whi3 and Pub1 to colocalize in distinct cytoplasmic foci. Readdition of glucose caused foci to dissolve. Scale bar represents 5 µm. (B) Treatment with cycloheximide (CHX) inhibits stress granule formation by preventing polysome disassembly. Unlike cells treated with drug vehicle alone, cells treated with CHX form neither Pub1-mCh nor Whi3-GFP foci. (C) Cells lacking the scaffolding protein eIF4GII do not form stress granules. Neither Whi3-mCh nor Pab1-GFP forms foci in <i>eif4gii</i>Δ cells. (D) Stress granule marker Pub1-mCh is still able to localize to foci in the absence of Whi3.</p>