10.1371/journal.pone.0084060.g001
Kristen J. Holmes
Kristen
J. Holmes
Daniel M. Klass
Daniel
M. Klass
Evan L. Guiney
Evan
L. Guiney
Martha S. Cyert
Martha
S. Cyert
Whi3 localizes to stress granules during glucose deprivation.
Public Library of Science
2013
Biochemistry
Nucleic acids
rna
Macromolecular assemblies
genetics
Gene function
Molecular genetics
Model organisms
Yeast and fungal models
Saccharomyces cerevisiae
Molecular cell biology
Cellular stress responses
Cellular structures
gene expression
localizes
granules
glucose
2013-12-27 02:42:57
Figure
https://plos.figshare.com/articles/figure/_Whi3_localizes_to_stress_granules_during_glucose_deprivation_/887418
<p>(A) Cells coexpressing Whi3-GFP and stress granule marker Pub1-mCherry were deprived of glucose for 10 minutes. Glucose deprivation caused Whi3 and Pub1 to colocalize in distinct cytoplasmic foci. Readdition of glucose caused foci to dissolve. Scale bar represents 5 µm. (B) Treatment with cycloheximide (CHX) inhibits stress granule formation by preventing polysome disassembly. Unlike cells treated with drug vehicle alone, cells treated with CHX form neither Pub1-mCh nor Whi3-GFP foci. (C) Cells lacking the scaffolding protein eIF4GII do not form stress granules. Neither Whi3-mCh nor Pab1-GFP forms foci in <i>eif4gii</i>Δ cells. (D) Stress granule marker Pub1-mCh is still able to localize to foci in the absence of Whi3.</p>