A. Ferguson, Annabel Roy, Sudipa N. Kormanik, Kaitlyn Kim, Yongsoon J. Dumas, Kathleen B. Ritov, Vladimir Matern, Dietrich J. Hu, Patrick L. Fisher, Alfred Tyrosine aminotransferase mutations enhance <i>eak</i> dauer and lifespan phenotypes. <p>(A) Effect of treatment of <i>eri-3(mg408); eak-4(mg348)</i> mutants with RNAi clones for <i>daf-2</i>, <i>ftt-2</i>, <i>akt-1</i>, <i>daf-7</i>, <i>daf-9</i>, <i>daf-11</i>, and <i>tatn-1</i> on dauer arrest at 25°C. Bars represent mean percentage of dauers observed in two trials, and the error bars represent standard deviation. The empty vector RNAi vector was used as a negative control. (B) Enhanced dauer formation by <i>eak-4(mg348);tatn-1(baf1)</i> versus the <i>eak-4(mg348)</i> or <i>tatn-1(baf1)</i> mutations alone. ** p<0.001 for pairwise Fisher's exact test. (C) <i>tatn-1</i> mutations enhance dauer arrest by <i>eak-3(mg344)</i>, <i>sdf-9(mg337)</i>, and <i>hsd-1(mg337)</i> mutants. ** p<0.001 for each double mutant compared to its respective <i>eak</i> single mutant by Fisher's exact test. (D) <i>tatn-1</i> enhances <i>eak-7(tm3188)</i> dauer arrest. ** p<0.001 for comparisons between <i>eak-7;tatn-1</i> and either <i>tatn-1</i> or <i>eak-7</i> alone. (E) <i>tatn-1(baf1)</i> extends the lifespan of both wild type and <i>eak-7</i> worms. Shown are survival curves for wild type N2, <i>tatn-1(baf1)</i>, <i>eak-7(3188)</i>, and <i>eak-7(3188); tatn-1(baf1)</i>, with error bars showing the 95% confidence intervals for each point. The mean survival for N2, <i>tatn-1</i>, <i>eak-7</i>, and <i>eak-7;tatn-1</i> are 21.0, 23.0, 30.0, and 33.5 days, respectively. p<0.001 for all comparisons by log-rank test.</p> aminotransferase;mutations;dauer;lifespan 2013-12-19
    https://plos.figshare.com/articles/figure/_Tyrosine_aminotransferase_mutations_enhance_eak_dauer_and_lifespan_phenotypes_/882409
10.1371/journal.pgen.1004020.g002