10.1371/journal.pone.0083324.g005 Shintaro Seto Shintaro Seto Keiko Sugaya Keiko Sugaya Kunio Tsujimura Kunio Tsujimura Toshi Nagata Toshi Nagata Toshinobu Horii Toshinobu Horii Yukio Koide Yukio Koide Involvement of Caspase-1 and classical autophagy pathway in autophagy induced by LPS Public Library of Science 2013 caspase-1 autophagy pathway induced 2013-12-13 03:04:11 Figure https://plos.figshare.com/articles/figure/_Involvement_of_Caspase_1_and_classical_autophagy_pathway_in_autophagy_induced_by_LPS_/877439 <p>(A) Autophagy induced by LSP in Caspase-1-KD macrophages. The proportion of macrophages with LC3-dot (upper panel) and immunoblot analysis of LC3 processing (lower panel) are shown. (B) Autophagy induced by LSP in Atg5-KD macrophages. The proportion of macrophages with LC3-dot (upper panel) and immunoblot analysis of LC3 processing (lower panel) are shown. (C) Autophagy induced by LPS in the presence of a PI3K inhibitor, 3-MA. Transfected macrophages were stimulated by LPS in the presence or absence of 3-MA at 10 mM for 24 h. The proportion of macrophages with LC3-dot (upper panel) and immunoblot analysis of LC3 processing (lower panel) are shown. Data represent the mean and SD of three or four independent experiments. *<i>p</i> < 0.05 (unpaired Student’s <i>t</i>-test).</p>