PA causes neuronal chromatin condensation <i>in vitro</i>. Ulrikke Voss Elin Sand Björn Olde Eva Ekblad 10.1371/journal.pone.0081413.g004 https://plos.figshare.com/articles/figure/_PA_causes_neuronal_chromatin_condensation_in_vitro_/867581 <p>Presence of neuronal chromatin condensation <i>in vitro</i> after PA treatment (10<sup>−4</sup> – 10<sup>−3</sup> M) compared to control (0). Upper panel: PA increases the number of neurons displaying nuclear chromatin condensation in a concentration-dependent manner. Lower panel: representative micrographs of HuC/HuD immunolabeled neurons (A–D) and Hoechst labeled nuclei (E–H) after control and PA (10<sup>−4</sup> M – 10<sup>−3</sup> M) treatment. Increasing concentrations of PA cause shrinkage of neuronal soma and nuclei and increase chromatin condensation (revealed as white spots in Hoechst stain). (A, E) control, (B, F) PA (10<sup>−4</sup> M), (C, G) PA (4×10<sup>−4</sup> M) and (D, H) PA (10<sup>−3</sup> M). Data shown as mean ± SEM, *** p<0.001, n = 5, Bar represents 20 µm.</p> 2013-12-03 03:54:55 causes neuronal chromatin condensation