%0 Figure %A Wang, Chunchao %A Wei, Qiang %A Zhang, Kang %A Wang, Ling %A Liu, Fengxia %A Zhao, Linna %A Tan, Yuanjun %A Di, Chao %A Yan, Hong %A Yu, Jingjuan %A Sun, Chuanqing %A J. Chen, Wenqiong %A Xu, Wenying %A Su, Zhen %D 2013 %T Real-time RT-PCR validation for selected probe sets. %U https://plos.figshare.com/articles/figure/_Real_time_RT_PCR_validation_for_selected_probe_sets_/867563 %R 10.1371/journal.pone.0081849.g004 %2 https://plos.figshare.com/ndownloader/files/1302231 %K rt-pcr %K validation %K probe %X

Twelve genes were selected for real-time RT-PCR to validate the expression patterns of Ubi::OsSPX1-antisense transgenic lines (A1 and A2) and WT rice (Ni) seedlings under control condition and cold treatment. These genes are: LOC_Os05g48390 - OsPHO2, ubiquitin conjugating enzyme; LOC_Os10g38600 - glutathione S-transferase GSTU6; LOC_Os10g38540 - glutathione S-transferase GSTU6; LOC_Os10g38350 - glutathione S-transferase GSTU6; LOC_Os01g72150 - glutathione S-transferase; LOC_Os01g72140 - glutathione S-transferase; LOC_Os01g43774 - cytochrome P450 CYP72A23; LOC_Os07g23570 - cytochrome P450 CYP709C9; LOC_Os09g25070 - OsWRKY62; LOC_Os02g08440 - OsWRKY71; LOC_Os06g37300 - ent-kaurene oxidase; LOC_Os11g47500 - xylanase inhibitor protein 1 precursor. The error bars of real-time RT-PCR results represent the standard error from three independent biological replicates.

%I PLOS ONE