10.1371/journal.pone.0218920.g001
Zeljko Grubac
Zeljko
Grubac
Nikola Sutulovic
Nikola
Sutulovic
Anida Ademovic
Anida
Ademovic
Milica Velimirovic
Milica
Velimirovic
Aleksandra Rasic-Markovic
Aleksandra
Rasic-Markovic
Djuro Macut
Djuro
Macut
Natasa Petronijevic
Natasa
Petronijevic
Olivera Stanojlovic
Olivera
Stanojlovic
Dragan Hrncic
Dragan
Hrncic
Schematic illustration of experimental design time flow.
Public Library of Science
2019
progesterone
Results SF rats
estradiol
anxiety-related behavior
OFF mode
fragmentation
TC
AC
30 min OFF mode
2019-07-03 20:05:23
Figure
https://plos.figshare.com/articles/figure/Schematic_illustration_of_experimental_design_time_flow_/8636858
<p>Animals (n = 42) were acclimatized to the laboratory conditions (7 days). Adaptation to the treadmill was performed in sessions of 1h (5 min ON and 5 min OFF working regime, treadmill belt speed ON = 0.02 m/s, OFF = 0m/s) during the next 3 days. Afterwards, the sleep fragmentation protocol has been applied and animals were divided into three groups (n = 14 in each group) and subjected to one of the three protocols in the treadmill during 6h of the light period: 1) sleep fragmentation (30s OFF: 90 s ON regime, 30 sleep interruptions per hour, SF group), 2) activity control group (10min ON: 30 min OFF regime, 1.5 sleep interruptions per hour, AC group), 3) treadmill control group (constantly OFF regime, 0 sleep interruptions per hour, TC group). Upon completion of sleep fragmentation protocols one cohort of animals (n = 8 from each group) has been subjected to in vivo ethological test of anxiety-like behavior (open filed, light—dart and elevated plus maze test), while another cohort of animals (n = 6 from each group) was sacrificed for blood sampling and further in vitro ELISA determination of hormonal levels (testosterone, progesterone, estradiol, corticosterone). For further details see the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0218920#sec006" target="_blank">Materials and Methods</a> section.</p>