mTORC2 regulates invasion of bladder cancer cells into the human bladder wall. GuptaSounak HauAndrew M. R. BeachJordan HarwalkerJyoti MantuanoElisabetta L. GoniasSteven T. EgelhoffThomas E. HanselDonna 2013 <p><i>A</i>, schematic showing orientation of human bladder wall invasion assay that includes lamina propria (LP), muscularis propria (MP) and perivesical fat (PV fat). B, H&E section showing invading J82 cells in the lamina propria after 72 h (scale bar 200 microns) <i>C</i>, cells highlighted in previous panel showed positive immunostaining for pancytokeratin (scale bar 100 microns). <i>D</i>, immunofluorescent stain shows invasion of malignant J82 cells (red), at 72 hr (scale bar 50 microns). <i>E</i>, quantification of tissue invasion by RT4 and J82 cells (microns). <i>F</i>, rictor gene-silencing significantly inhibits invasion of bladder all by J82 cells. (*) statistically significant comparison using student t-test, p<0.05).</p>