Bioluminescence and weight loss in directly inoculated mice. Crystal W. Burke Olga Bridges Sherri Brown Richard Rahija Charles J. Russell 10.1371/journal.ppat.1003786.g002 https://plos.figshare.com/articles/figure/_Bioluminescence_and_weight_loss_in_directly_inoculated_mice_/859950 <p>129X1 mice were inoculated intranasally with 70- (<b>A</b>) or 7,000- (<b>B</b>) PFU of rSeV-luc(M-F*), or PBS (<b>C</b>) and bioluminescence in the nasopharynx (red triangles), trachea (orange circles), and lungs (blue squares) was measured for 14 days. Seventy days after the initial inoculation, the same mice were challenge with a lethal dose of 3×10<sup>6</sup>-PFU of rSeV-luc(M-F*) and bioluminescence was again measured daily. (<b>D</b>) Weight loss was used as a measure of morbidity and was monitored throughout the course of experiment. All numbers are reported as the means ± the standard deviation (70-PFU <i>n</i> = 22 and 7,000-PFU <i>n</i> = 48). The bottom of the y-axis is 5.5×10<sup>5</sup> photons/s, the limit of detection of bioluminescence.</p> 2013-11-21 04:38:20 inoculated