10.1371/journal.pone.0218507.g004
Rohana P. Dassanayake
Rohana P.
Dassanayake
Shollie M. Falkenberg
Shollie M.
Falkenberg
Eric M. Nicholson
Eric
M. Nicholson
Robert E. Briggs
Robert
E. Briggs
Fred M. Tatum
Fred M.
Tatum
Vijay K. Sharma
Vijay
K. Sharma
Timothy A. Reinhardt
Timothy A.
Reinhardt
Flow cytometric analysis confirmed that both bNK2A peptides induced damage to <i>Histophilus somni</i> membranes.
Public Library of Science
2019
well-conserved cysteine residues
bNK 2A antimicrobial activity
30- mer bNK 2A peptides
intra-chain disulfide bonds
bNK 2A peptide
Mature NK-lysin protein
bactericidal activity Bovine NK-lysins
disulfide bonds
bNK 2A forms disulfide bonds
α- helical structures
NK-lysin-derived peptides show antimicrobial activity
NK-lysin-derived peptide
cationic antimicrobial proteins
2019-06-19 17:37:25
Figure
https://plos.figshare.com/articles/figure/Flow_cytometric_analysis_confirmed_that_both_bNK2A_peptides_induced_damage_to_i_Histophilus_somni_i_membranes_/8297249
<p>Live and dead <i>H</i>. <i>somni</i> in PBS control (A, PBS), heat-killed at 56°C for 10 min (B), and treated with 20 μM bNK2A-C10C20 (C), or 20 μM bNK2A-S10S20 (D) at 37°C for 30 min were stained with LIVE/DEAD <i>Bac</i>Light bacterial viability dyes. X-axis indicates live bacteria (Syto 9) and Y-axis indicates dead bacteria (propidium iodide). Results of one representative experiment out of three are shown.</p>