10.1371/journal.pone.0218507.g004 Rohana P. Dassanayake Rohana P. Dassanayake Shollie M. Falkenberg Shollie M. Falkenberg Eric M. Nicholson Eric M. Nicholson Robert E. Briggs Robert E. Briggs Fred M. Tatum Fred M. Tatum Vijay K. Sharma Vijay K. Sharma Timothy A. Reinhardt Timothy A. Reinhardt Flow cytometric analysis confirmed that both bNK2A peptides induced damage to <i>Histophilus somni</i> membranes. Public Library of Science 2019 well-conserved cysteine residues bNK 2A antimicrobial activity 30- mer bNK 2A peptides intra-chain disulfide bonds bNK 2A peptide Mature NK-lysin protein bactericidal activity Bovine NK-lysins disulfide bonds bNK 2A forms disulfide bonds α- helical structures NK-lysin-derived peptides show antimicrobial activity NK-lysin-derived peptide cationic antimicrobial proteins 2019-06-19 17:37:25 Figure https://plos.figshare.com/articles/figure/Flow_cytometric_analysis_confirmed_that_both_bNK2A_peptides_induced_damage_to_i_Histophilus_somni_i_membranes_/8297249 <p>Live and dead <i>H</i>. <i>somni</i> in PBS control (A, PBS), heat-killed at 56°C for 10 min (B), and treated with 20 μM bNK2A-C10C20 (C), or 20 μM bNK2A-S10S20 (D) at 37°C for 30 min were stained with LIVE/DEAD <i>Bac</i>Light bacterial viability dyes. X-axis indicates live bacteria (Syto 9) and Y-axis indicates dead bacteria (propidium iodide). Results of one representative experiment out of three are shown.</p>