10.1371/journal.pone.0217679
Jun Mu
Jun
Mu
Xia Cui
Xia
Cui
Mingjiao Shao
Mingjiao
Shao
Yuxia Wang
Yuxia
Wang
Qiao Yang
Qiao
Yang
Guangfeng Yang
Guangfeng
Yang
Liying Zheng
Liying
Zheng
Microbial origin of bioflocculation components within a promising natural bioflocculant resource of <i>Ruditapes philippinarum</i> conglutination mud from an aquaculture farm in Zhoushan, China
Public Library of Science
2019
EPS
flocculation-effective RPM polysaccharides
MWH
Bacillus sp
RPM bioflocculation components
bivalve mollusk R
RPM flocculation components
aquaculture farm
bioflocculation components
CL
Ruditapes philippinarum conglutination mud
bioflocculant resource
bioflocculant-producing strains
China Ruditapes philippinarum conglutination mud
2019-06-19 17:32:31
Dataset
https://plos.figshare.com/articles/dataset/Microbial_origin_of_bioflocculation_components_within_a_promising_natural_bioflocculant_resource_of_i_Ruditapes_philippinarum_i_conglutination_mud_from_an_aquaculture_farm_in_Zhoushan_China/8296550
<div><p><i>Ruditapes philippinarum</i> conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk <i>R</i>. <i>philippinarum</i> and has been recently reported as a promising natural bioflocculant resource. However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation. This study investigated the probability that RPM bioflocculation components originate from its associated microbes. RPM samples from an aquaculture farm in Zhoushan of China were applied to characterize its microbial community structure, screen associated bioflocculant-producing strains, and explore the homology between extracellular polysaccharides (EPS) from bioflocculant-producing isolates and RPM flocculation components. Results showed that RPM exhibited high bacterial biodiversity, with Proteobacteria, Bacteroidetes and Actinobacteria as the most abundant phyla; <i>hgcI_clade</i>, <i>CL500_29_marine_group</i>, <i>Fusibacter</i>, <i>MWH_UniP1_aquatic_group</i> and <i>Arcobacter</i> as the dominant genera. Fourteen highly efficient bioflocculant-producing strains were screened and phylogenetically identified as <i>Pseudoalteromonas</i> sp. (5), <i>Psychrobacter</i> sp. (3), <i>Halomonas</i> sp. (2), <i>Albirhodobacter</i> sp. (1), <i>Celeribacter</i> sp. (1), <i>Kocuria</i> sp. (1) and <i>Bacillus</i> sp. (1), all of which except <i>Bacillus</i> sp. were reported for the first time for their excellent flocculation capability. Furthermore, EPS from the bioflocculant-producing strains exhibited highly similar monosaccharide composition to the reported flocculation-effective RPM polysaccharides. On the other hand, the existence of fungi in RPM was rare and showed no flocculation functionality. Findings from Zhoushan RPM strongly supported that RPM flocculation components were of bacterial origin and make RPM reproduction possible by fermentation approach.</p></div>