M. M. Gommers, Lisanne Hill, Thomas G. Ashcroft, Frances M. H. F. de Baaij, Jeroen Low extracellular magnesium does not impair glucose-stimulated insulin secretion <div><p>There is an increasing amount of clinical evidence that hypomagnesemia (serum Mg<sup>2+</sup> levels < 0.7 mmol/l) contributes to type 2 diabetes mellitus pathogenesis. Amongst other hypotheses, it has been suggested that Mg<sup>2+</sup> deficiency affects insulin secretion. The aim of this study was, therefore, to investigate the acute effects of extracellular Mg<sup>2+</sup> on glucose-stimulated insulin secretion in primary mouse islets of Langerhans and the rat insulinoma INS-1 cell line. Here we show that acute lowering of extracellular Mg<sup>2+</sup> concentrations from 1.0 mM to 0.5 mM did not affect glucose-stimulated insulin secretion in islets or in insulin-secreting INS-1 cells. The expression of key genes in the insulin secretory pathway (e.g. <i>Gck</i>, <i>Abcc8)</i> was also unchanged in both experimental models. Knockdown of the most abundant Mg<sup>2+</sup> channel <i>Trpm7</i> by siRNAs in INS-1 cells resulted in a 3-fold increase in insulin secretion at stimulatory glucose conditions compared to mock-transfected cells. Our data suggest that insulin secretion is not affected by acute lowering of extracellular Mg<sup>2+</sup> concentrations.</p></div> Low extracellular magnesium;glucose-stimulated insulin secretion;Mg;insulin secretion;insulin secretory pathway;rat insulinoma INS;type 2 diabetes mellitus pathogenesis 2019-06-04
    https://plos.figshare.com/articles/dataset/Low_extracellular_magnesium_does_not_impair_glucose-stimulated_insulin_secretion/8225897
10.1371/journal.pone.0217925