Caveolin-1 and cholesterol dependent viral entry. Mohammad A. Yousuf Xiaohong Zhou Santanu Mukherjee Ashish V. Chintakuntlawar Jeong Yoon Lee Mirja Ramke James Chodosh Jaya Rajaiya 10.1371/journal.pone.0077462.g004 https://plos.figshare.com/articles/figure/_Caveolin_1_and_cholesterol_dependent_viral_entry_/820440 <p>siRNA and scrambled (sc)RNA were generated and transfected into human corneal cells. <b>A</b>. Real-time RT-PCR for caveolin-1 mRNA confirmed successful reduction of caveolin-1 message (~95%) as compared to scRNA transfected cells (*p=.0002). <b>B</b>. Western blot showed reduced expression of caveolin-1 in caveolin-1 specific siRNA treated cells (right lane) as compared to untransfected and uninfected cells (left lane) or scRNA transfected, virus infected cells (middle lane) <b>C</b>. IL-8 mRNA expression after HAdV-D37 infection was reduced to almost 50% by caveolin-1 siRNA transfection, compared to scRNA (*p=.0001). </p> 2013-10-11 03:02:09 viral