Transfection of HEK293FT cells with pEGFP with varying ratios of PLGA.
Eun Jin Seo
Il Ho Jang
Eun Kyoung Do
Hyo Cheon Cheon
Soon Chul Heo
Yang Woo Kwon
Geun Ok Jeong
Ba Reun Kim
Jae Ho Kim
10.1371/journal.pone.0076875.g004
https://plos.figshare.com/articles/figure/_Transfection_of_HEK293FT_cells_with_pEGFP_with_varying_ratios_of_PLGA_/810532
<p>(A) Fluorescence and bright field images (B) flow cytometry analysis of cells at 48 h after transfection with varying ratios of PLGA/bPEI-DNA (PLGA:bPEI:DNA, w/w/w) nanoparticles are shown. (C) Results of gel electrophoresis analysis of bPEI-DNA (bPEI:DNA, w/w, upper panel) and PLGA/bPEI-DNA (PLGA:bPEI:DNA, w/w/w, lower panel) nanoparticles are shown. 1 µg of DNA was applied to each well (transfection, six-well plate) or lane. Scale bar represents 100 µm (*, p < 0.05 by Student’s t-test). n=3.</p>
2013-09-30 01:31:11
hek293ft
cells
pegfp
varying
ratios