IC<sub>50</sub> determination of hexose uptake by HEK293 cells overexpressing representative human class II and III orthologues. HeitmeierMonique R. C. HreskoRichard EdwardsRachel L. PrinsenMichael J. IlaganMa Xenia G. Odom JohnAudrey R. W. HruzPaul 2019 <p>A) Uptake of 2-DG by HEK293 cells overexpressing either PfHT or class III GLUT transporter, hGLUT8. B) Uptake of [<sup>3</sup>H]-D-fructose (Frc) in HEK293 cells overexpressing hGLUT8, or PfHT or class II transporter, GLUT5. Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate. IC<sub>50</sub>s were calculated using nonlinear regression analysis (GraphPad Prism 6.0) and are tabulated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0216457#pone.0216457.t002" target="_blank">Table 2</a>.</p>