Heitmeier, Monique R. C. Hresko, Richard Edwards, Rachel L. Prinsen, Michael J. Ilagan, Ma Xenia G. Odom John, Audrey R. W. Hruz, Paul Inhibition of growth and IC<sub>50</sub> determination of glucose uptake by isolated <i>P</i>. <i>falciparum</i> parasites. <p>A) IC<sub>50</sub>s for inhibition of growth of <i>P</i>. <i>falciparum</i> strain 3D7 intraerythrocytic forms by each compound were determined via a growth inhibition assay as described in Materials and Methods. Data are expressed as means ± SEMs of three independent experiments performed in duplicate. B) Uptake of 2-DG by isolated <i>P</i>. <i>falciparum</i> trophozoites at increasing concentrations of WU-1. Distribution ratios (i.e., the ratio of intracellular concentration of radiolabel relative to the extracellular concentration) were calculated as described previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0216457#pone.0216457.ref023" target="_blank">23</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0216457#pone.0216457.ref050" target="_blank">50</a>]. IC<sub>50</sub>s were calculated using nonlinear regression analysis (GraphPad Prism 6.0). Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate.</p> zero-trans D-fructose uptake;WU;endofacial ligand cytochalasin B;Maybridge HitFinder chemical library;facilitative hexose transporters;10 μ M;antagonist;N-terminal FLAG tag;PfHT over-expressing cell lines;Plasmodium falciparum hexose transporter PfHT;GLUT;EC;ATB-BMPA;FLAG-mediated protein engineering;glucose permeation pathway;2- DG uptake;glucose transport inhibitors;IC 50;throughput screening platform;III;II 2019-05-09
    https://plos.figshare.com/articles/figure/Inhibition_of_growth_and_IC_sub_50_sub_determination_of_glucose_uptake_by_isolated_i_P_i_i_falciparum_i_parasites_/8103326
10.1371/journal.pone.0216457.g004