%0 Figure %A Heitmeier, Monique R. %A C. Hresko, Richard %A Edwards, Rachel L. %A Prinsen, Michael J. %A Ilagan, Ma Xenia G. %A Odom John, Audrey R. %A W. Hruz, Paul %D 2019 %T Inhibition of growth and IC50 determination of glucose uptake by isolated P. falciparum parasites. %U https://plos.figshare.com/articles/figure/Inhibition_of_growth_and_IC_sub_50_sub_determination_of_glucose_uptake_by_isolated_i_P_i_i_falciparum_i_parasites_/8103326 %R 10.1371/journal.pone.0216457.g004 %2 https://plos.figshare.com/ndownloader/files/15115832 %K zero-trans D-fructose uptake %K WU %K endofacial ligand cytochalasin B %K Maybridge HitFinder chemical library %K facilitative hexose transporters %K 10 μ M %K antagonist %K N-terminal FLAG tag %K PfHT over-expressing cell lines %K Plasmodium falciparum hexose transporter PfHT %K GLUT %K EC %K ATB-BMPA %K FLAG-mediated protein engineering %K glucose permeation pathway %K 2- DG uptake %K glucose transport inhibitors %K IC 50 %K throughput screening platform %K III %K II %X

A) IC50s for inhibition of growth of P. falciparum strain 3D7 intraerythrocytic forms by each compound were determined via a growth inhibition assay as described in Materials and Methods. Data are expressed as means ± SEMs of three independent experiments performed in duplicate. B) Uptake of 2-DG by isolated P. falciparum trophozoites at increasing concentrations of WU-1. Distribution ratios (i.e., the ratio of intracellular concentration of radiolabel relative to the extracellular concentration) were calculated as described previously [23, 50]. IC50s were calculated using nonlinear regression analysis (GraphPad Prism 6.0). Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate.

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