IC<sub>50</sub> determination of glucose uptake by HEK293 cells overexpressing human class I orthologues. Monique R. Heitmeier Richard C. Hresko Rachel L. Edwards Michael J. Prinsen Ma Xenia G. Ilagan Audrey R. Odom John Paul W. Hruz 10.1371/journal.pone.0216457.g003 https://plos.figshare.com/articles/figure/IC_sub_50_sub_determination_of_glucose_uptake_by_HEK293_cells_overexpressing_human_class_I_orthologues_/8103323 <p>2-DG uptake in response to increasing WU-1 (A) or WU-2 (B) concentrations in HEK293 cells expressing the indicated GLUT protein. IC<sub>50</sub>s were calculated using nonlinear regression analysis using GraphPad Prism 6.0 and are tabulated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0216457#pone.0216457.t001" target="_blank">Table 1</a>. Uptake data are expressed as means ± SEMs of three independent experiments performed in triplicate.</p> 2019-05-09 17:44:05 zero-trans D-fructose uptake WU endofacial ligand cytochalasin B Maybridge HitFinder chemical library facilitative hexose transporters 10 μ M antagonist N-terminal FLAG tag PfHT over-expressing cell lines Plasmodium falciparum hexose transporter PfHT GLUT EC ATB-BMPA FLAG-mediated protein engineering glucose permeation pathway 2- DG uptake glucose transport inhibitors IC 50 throughput screening platform III II