The linker region of DRE2 coordinates protein-protein interactions in the CIA pathway.
Xiaokang Wang
Xudong Chen
Linhua Sun
Weiqiang Qian
10.1371/journal.pgen.1008094.g004
https://plos.figshare.com/articles/figure/The_linker_region_of_DRE2_coordinates_protein-protein_interactions_in_the_CIA_pathway_/8053883
<p>(A-B) Interactions of DRE2, DRE2<sup><i>Δ</i>6</sup>, DRE2-3 and DRE2<sup><i>Δ</i>75</sup> with TAH18, NBP35 and GRXS17 as determined by Y2H assays. Yeast cells harboring different fusion protein combinations (listed at the left) in pGBK-T7 (BD) and pGAD-T7 (AD) vectors were plated on medium lacking Leu, Trp (SD-TL), medium lacking Leu, Trp and His (SD-TLH) and medium lacking Leu, Trp, His and Ade (SD-TLHA) (A) or SD-TLH medium with 1 mM 3AT (B).</p>
2019-04-29 17:26:20
dre 2 mutants exhibit DNA hypermethylation
CIA-related protein interactions
dre 2 mutants
DRE 2 associates
CIA pathway mutants
linker region
Canonical cytosolic iron-sulfur cluster assembly
auxin response
show constitutive DNA damage response
NBP
Cytosolic Iron-sulfur cluster Assembly
DRE 2 localizes
DRE 2
CRISPR
hypomorphic dre 2 mutants
GRXS