10.1371/journal.pgen.1008094.g004 Xiaokang Wang Xiaokang Wang Xudong Chen Xudong Chen Linhua Sun Linhua Sun Weiqiang Qian Weiqiang Qian The linker region of DRE2 coordinates protein-protein interactions in the CIA pathway. Public Library of Science 2019 dre 2 mutants exhibit DNA hypermethylation CIA-related protein interactions dre 2 mutants DRE 2 associates CIA pathway mutants linker region Canonical cytosolic iron-sulfur cluster assembly auxin response show constitutive DNA damage response NBP Cytosolic Iron-sulfur cluster Assembly DRE 2 localizes DRE 2 CRISPR hypomorphic dre 2 mutants GRXS 2019-04-29 17:26:20 Figure https://plos.figshare.com/articles/figure/The_linker_region_of_DRE2_coordinates_protein-protein_interactions_in_the_CIA_pathway_/8053883 <p>(A-B) Interactions of DRE2, DRE2<sup><i>Δ</i>6</sup>, DRE2-3 and DRE2<sup><i>Δ</i>75</sup> with TAH18, NBP35 and GRXS17 as determined by Y2H assays. Yeast cells harboring different fusion protein combinations (listed at the left) in pGBK-T7 (BD) and pGAD-T7 (AD) vectors were plated on medium lacking Leu, Trp (SD-TL), medium lacking Leu, Trp and His (SD-TLH) and medium lacking Leu, Trp, His and Ade (SD-TLHA) (A) or SD-TLH medium with 1 mM 3AT (B).</p>