10.1371/journal.pgen.1008094.g003
Xiaokang Wang
Xiaokang
Wang
Xudong Chen
Xudong
Chen
Linhua Sun
Linhua
Sun
Weiqiang Qian
Weiqiang
Qian
Dysfunction of <i>DRE2</i> causes genome-wide DNA hypermethylation.
Public Library of Science
2019
dre 2 mutants exhibit DNA hypermethylation
CIA-related protein interactions
dre 2 mutants
DRE 2 associates
CIA pathway mutants
linker region
Canonical cytosolic iron-sulfur cluster assembly
auxin response
show constitutive DNA damage response
NBP
Cytosolic Iron-sulfur cluster Assembly
DRE 2 localizes
DRE 2
CRISPR
hypomorphic dre 2 mutants
GRXS
2019-04-29 17:26:20
Figure
https://plos.figshare.com/articles/figure/Dysfunction_of_i_DRE2_i_causes_genome-wide_DNA_hypermethylation_/8053880
<p>(A) Venn diagram showing the numbers of hyper-DMRs that either overlap between or are unique in <i>dre2-4</i> and <i>ros1-4</i>. Boxplots showing the distribution and the average CG, CHG, or CHH methylation levels calculated from hyper-DMRs in the respective subgroups. Dark horizontal line, median; edges of boxes, 25th (bottom) and 75th (top) percentiles; whiskers, minimum and maximum percentage of DNA methylation. (B) Heat-map showing the methylation levels of <i>dre2-4</i>-associated hyper-DMRs in Col-0, <i>dre2-4</i>, <i>met18-2</i> and <i>ros1-4</i>. The color key is presented at right. Light yellow indicates low methylation and black indicates high methylation. (C) Locus-specific bisulfite sequencing results showing the DNA methylation levels of the <i>ROS1</i> promoter in different genotypes. (D) Relative expression levels of <i>ROS1</i> in the indicated genotypes as determined by RT-qPCR. Asterisks indicate two-tailed Student’s t-test, *P < 0.05, **P < 0.01. Results from the second biological replicate are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008094#pgen.1008094.s005" target="_blank">S5D Fig</a>.</p>