10.1371/journal.pgen.1008094.g003 Xiaokang Wang Xiaokang Wang Xudong Chen Xudong Chen Linhua Sun Linhua Sun Weiqiang Qian Weiqiang Qian Dysfunction of <i>DRE2</i> causes genome-wide DNA hypermethylation. Public Library of Science 2019 dre 2 mutants exhibit DNA hypermethylation CIA-related protein interactions dre 2 mutants DRE 2 associates CIA pathway mutants linker region Canonical cytosolic iron-sulfur cluster assembly auxin response show constitutive DNA damage response NBP Cytosolic Iron-sulfur cluster Assembly DRE 2 localizes DRE 2 CRISPR hypomorphic dre 2 mutants GRXS 2019-04-29 17:26:20 Figure https://plos.figshare.com/articles/figure/Dysfunction_of_i_DRE2_i_causes_genome-wide_DNA_hypermethylation_/8053880 <p>(A) Venn diagram showing the numbers of hyper-DMRs that either overlap between or are unique in <i>dre2-4</i> and <i>ros1-4</i>. Boxplots showing the distribution and the average CG, CHG, or CHH methylation levels calculated from hyper-DMRs in the respective subgroups. Dark horizontal line, median; edges of boxes, 25th (bottom) and 75th (top) percentiles; whiskers, minimum and maximum percentage of DNA methylation. (B) Heat-map showing the methylation levels of <i>dre2-4</i>-associated hyper-DMRs in Col-0, <i>dre2-4</i>, <i>met18-2</i> and <i>ros1-4</i>. The color key is presented at right. Light yellow indicates low methylation and black indicates high methylation. (C) Locus-specific bisulfite sequencing results showing the DNA methylation levels of the <i>ROS1</i> promoter in different genotypes. (D) Relative expression levels of <i>ROS1</i> in the indicated genotypes as determined by RT-qPCR. Asterisks indicate two-tailed Student’s t-test, *P < 0.05, **P < 0.01. Results from the second biological replicate are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008094#pgen.1008094.s005" target="_blank">S5D Fig</a>.</p>