Inhibition of the Th2 response with anti-IL-4 antibodies does not confer anakinra or Dex resistance in IL-17A production by antigen-restimulated lung cells in NO<sub>2</sub>-promoted allergic airway disease. Rebecca A. Martin Jennifer L. Ather Rebecca Daggett Laura Hoyt John F. Alcorn Benjamin T. Suratt Daniel J. Weiss Lennart K. A. Lundblad Matthew E. Poynter 10.1371/journal.pone.0074730.g005 https://plos.figshare.com/articles/figure/_Inhibition_of_the_Th2_response_with_anti_IL_4_antibodies_does_not_confer_anakinra_or_Dex_resistance_in_IL_17A_production_by_antigen_restimulated_lung_cells_in_NO_2_promoted_allergic_airway_disease_/803447 <p>Mice were treated as in Figure 2. Lung single cell suspensions were restimulated with OVA antigen in the presence or absence of anakinra (Ana; 1 μg/mL), Dex (10<sup>-8</sup> M), or anakinra and Dex in combination for 96 hours. Cell supernatants from antigen-restimulated and treated lung cells from IgG isotype control treated mice (A-D) or anti-IL-4 treated mice (E-H) were analyzed for cytokines by ELISA. Statistics were performed by one-way ANOVA and Bonferroni post hoc analysis (E-L). **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05 compared to controls (none) unless otherwise indicated by brackets. ND, not significantly different compared to controls. n = 6-12 per group.</p> 2013-09-19 02:10:07 th2 anti-il-4 antibodies confer anakinra dex il-17a antigen-restimulated cells allergic airway