Inhibition of the Th2 response with anti-IL-4 antibodies does not confer anakinra or Dex resistance in IL-17A production by antigen-restimulated lung cells in NO<sub>2</sub>-promoted allergic airway disease.
Rebecca A. Martin
Jennifer L. Ather
Rebecca Daggett
Laura Hoyt
John F. Alcorn
Benjamin T. Suratt
Daniel J. Weiss
Lennart K. A. Lundblad
Matthew E. Poynter
10.1371/journal.pone.0074730.g005
https://plos.figshare.com/articles/figure/_Inhibition_of_the_Th2_response_with_anti_IL_4_antibodies_does_not_confer_anakinra_or_Dex_resistance_in_IL_17A_production_by_antigen_restimulated_lung_cells_in_NO_2_promoted_allergic_airway_disease_/803447
<p>Mice were treated as in Figure 2. Lung single cell suspensions were restimulated with OVA antigen in the presence or absence of anakinra (Ana; 1 μg/mL), Dex (10<sup>-8</sup> M), or anakinra and Dex in combination for 96 hours. Cell supernatants from antigen-restimulated and treated lung cells from IgG isotype control treated mice (A-D) or anti-IL-4 treated mice (E-H) were analyzed for cytokines by ELISA. Statistics were performed by one-way ANOVA and Bonferroni post hoc analysis (E-L). **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05 compared to controls (none) unless otherwise indicated by brackets. ND, not significantly different compared to controls. n = 6-12 per group.</p>
2013-09-19 02:10:07
th2
anti-il-4
antibodies
confer
anakinra
dex
il-17a
antigen-restimulated
cells
allergic
airway