Expression analysis of selected genes involved in carotenoid metabolism.
Angela Rubio Moraga
Oussama Ahrazem
José Luis Rambla
Antonio Granell
Lourdes Gómez Gómez
10.1371/journal.pone.0071946.g007
https://plos.figshare.com/articles/figure/_Expression_analysis_of_selected_genes_involved_in_carotenoid_metabolism_/798963
<p>A. Schematic carotenoid and apocarotenoid biosynthetic pathway in <i>Crocus</i>. Enzymatic reactions are represented by arrows. GGPP, geranyl geranyl diphosphate; PSY, phytoene synthase; PDS, phytoene desaturase; ZDS, z-carotene desaturase; CRTISO, carotene isomerase; LCYe, lycopene e-cyclase; LCYb, lycopene b-cyclase; bCH, b-carotene hydroxylase; eCH, e-carotene hydroxylase; ZEP, zeaxanthin epoxidase; VDE, violaxanthin de-epoxidase; NXS, neoxanthin synthase; CCD, carotenoid cleavage dioxygenase. B. RT-PCR analysis for expression of selected candidate genes related to the carotenoid metabolism. Equal amounts of total RNA were used in each reaction. The levels of constitutively expressed <i>RPS18</i> coding gene were assayed as controls. The PCR products were separated by 1.5% (w/v) agarose gel electrophoresis and visualized by ethidium bromide staining. C. Relative transcript levels in tepals of the genes analysed by RT-PCR in B.</p>
2013-09-13 01:39:34
genes
carotenoid