Overexpression of Mal3 (1–143) exhibits the TBZ-tolerance in the wild type strain. Takuma Tanabe Masayuki Yamaga Makoto Kawamukai Yasuhiro Matsuo 10.1371/journal.pone.0214803.g006 https://plos.figshare.com/articles/figure/Overexpression_of_Mal3_1_143_exhibits_the_TBZ-tolerance_in_the_wild_type_strain_/7983908 <p>(A) Wild type (PR109) strains harboring pREP3X (vector), pREP3X-mal3 (1–308; full-length), pREP3X-mal3 (1–143), or pREP3X-mal3 (135–308) were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0214803#pone.0214803.g001" target="_blank">Fig 1C</a>. Cells were streaked on EMMU in the presence or absence of 40 μg/mL TBZ or 60 μg/mL TBZ. All plates were incubated for 5 days at 30˚C. (B) <i>S</i>. <i>cerevisiae</i> AH109 strain was co-transformed with pGBKT7-derived plasmid and pGAD424-derived plasmid and selected on SC lacking leucine and tryptophan (SC-LW). Transformed cells were cultured and the cell suppression was spotted as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0214803#pone.0214803.g002" target="_blank">Fig 2B</a>. All plates were incubated for 3 days at 30˚C.</p> 2019-04-11 17:34:46 multi-copy suppressor cAMP-dependent protein kinase Pka 1 mal 3 gene 89E EB 1 orthologs microtubule-destabilization drug TBZ fission yeast pka 1 Mal 3 TBZ-sensitive phenotype chromosome mis-segregation Mal 3 CH domain Pka 1 high-affinity microtubule binding pka 1 gene chromosome segregation strain 89R Mal 3. Overexpression