10.1371/journal.pone.0074641.g004 Christina Greer Christina Greer Moonsook Lee Moonsook Lee Maaike Westerhof Maaike Westerhof Brandon Milholland Brandon Milholland Rebecca Spokony Rebecca Spokony Jan Vijg Jan Vijg Julie Secombe Julie Secombe Myc overexpression in the adult increases mutation frequency and reduces lifespan at 25 Public Library of Science 2013 overexpression mutation lifespan 2013-09-06 01:45:58 Figure https://plos.figshare.com/articles/figure/_Myc_overexpression_in_the_adult_increases_mutation_frequency_and_reduces_lifespan_at_25_/791153 <p>°<b>C.</b> (A) Western analyses showing levels of Myc and the loading control γ-tubulin from four female adult heads of the genotypes <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). Adults were placed at 25°C for 10 days before Western analyses. (B) <i>lacZ</i> mutation frequency of females of genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). Adults were placed at 25°C for 10 days before <i>lacZ</i> analyses. *indicates statistical significance of p<0.001 (student’s t-test). (C) Lifespan analyses at 25°C of females of the genotypes <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). These lifespans are statistically significantly different from each other p<0.001 (Log-rank test). (D) Lifespan analyses at 25°C of females of the genotypes <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-p35 (shown as “Act<sup>TS</sup>>p35”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc, UAS-p35 (shown as “Act<sup>TS</sup>>Myc+p35”). These are statistically significantly different (p<0.001, Log-rank test).</p>