Greer, Christina Lee, Moonsook Westerhof, Maaike Milholland, Brandon Spokony, Rebecca Vijg, Jan Secombe, Julie Overexpression of Myc increases mutation frequency and decreases lifespan at 29 <p>°<b>C.</b> (A) Western blot from five adult heads at 29°C for three days from <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as Act<sup>TS</sup>>+) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as Act<sup>TS</sup>>Myc). Myc is induced ∼9-fold in this system (LiCOR quantitation). (B) <i>lacZ</i> mutation frequency of <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). Adults were placed at 29°C for 5 days before <i>lacZ</i> analyses to allow transgene expression. Black solid areas indicate frequency of point mutations and white areas indicate frequency of genome rearrangements. *indicates statistical significance of p<0.01 (student’s t-test). (C) Lifespan analyses of females of the genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and of the genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). These lifespans are statistically significantly different from each other p<0.001 (Log-rank test).</p> myc;mutation;decreases;lifespan 2013-09-06
    https://plos.figshare.com/articles/figure/_Overexpression_of_Myc_increases_mutation_frequency_and_decreases_lifespan_at_29_/791152
10.1371/journal.pone.0074641.g003